Gotta pay to play fellas. If it's something that is proprietary to them, can't really blame them. Personally, if I am paying that much for an ECU AND a tune, you better not lock it...because you are getting into a standalone money territory.
From my experience, E85 leaves a very "clean" engine internally. You don't have your usual gum and coked oil deposits. That being said, your truck will have worse gas mileage and you need to double or even triple your oil change frequency simply because the oil dilution is higher. E85 by its...
Stupid ass people. I have seen it more times than I can count. Do not stop your vehicle in the middle of the road. Like seriously, use common sense, know when the vehicle is stalled and navigate your vehicle to the side of the road and turn your blinkers ON....and get OUT of the car IMMEDIATELY...
1000 miles and no more than 4000-5000 miles there after; Sooner, if on E85. My truck is currently at 9700 miles on the odo and has had 4 oil changes already.